CHIRALPAK® CBH HPLC Column
SUPELCO/58530AST - 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Product Type: Equipment/Book
| L × I.D. | 10 cm × 2.1 mm |
| manufacturer/tradename | CHIRALPAK® |
| material | stainless steel column |
| matrix | fully porous particle |
| matrix active group | cellobiohydrolase phase |
| operating pH | 2-8 |
| packaging | pkg of 1 ea |
| parameter | 137 bar pressure (2000 psi) |
| 20-30 °C temperature | |
| 40 °C max. temp. | |
| particle size | 5 μm |
| product line | CHIRALPAK® |
| Quality Level | 100  |
| separation technique | chiral |
| technique(s) | HPLC: suitable |
| General description: | Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. Used primarily for the separation enantiomers of basic compounds, CHIRALPAK CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles. Used in reversed-phase mode, retention and enantioselectivity is regulated by changes of pH, buffer concentration and organic modifier. The mobile phases are mixtures of phosphate or acetate buffers and organic solvents such as 2-propanol or acetonitrile. The column is preferably used for the separation of enantiomers of basic drugs, particularly compounds containing one or more nitrogen atoms along with one or more hydrogen-bonding groups (alcohol, phenol, carbonyl, amide, ether, ester, etc.). Features: • Direct reversed phase resolution of chiral molecules • Stable with a variety of organic modifiers • Available in analytical and semi-preparative sizes (1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80. |
| Legal Information: | CHIRALPAK is a registered trademark of Daicel Corp. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| UNSPSC | 41115700 |