Discovery® C8 HPLC Column
SUPELCO/59354-U - 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Synonym: C8 Reversed-Phase Chromatography Column
Product Type: Equipment/Book
| agency | suitable for USP L7 |
| application(s) | food and beverages |
| extent of labeling | 7.5% Carbon loading |
| feature | endcapped |
| impurities | <10 ppm metals |
| L × I.D. | 25 cm × 4.6 mm |
| manufacturer/tradename | Discovery® |
| material | stainless steel column |
| matrix | silica gel, high purity, spherical base material |
| fully porous particle | |
| matrix active group | C8 (octyl) phase |
| operating pH range | 2-8 |
| packaging | 1 ea of |
| parameter | ≤70 °C temp. range |
| 400 bar pressure (5801 psi) | |
| particle size | 5 μm |
| pore size | 180 Å |
| product line | Discovery® |
| Quality Level | 100  |
| separation technique | reversed phase |
| surface area | 200 m2/g |
| surface coverage | 3.4 μmol/m2 |
| technique(s) | HPLC: suitable |
| LC/MS: suitable |
| Application: | Discovery® C8 HPLC column may be used in the determination of bisphenol A, genistein, bisphenol A β-D-glucuronide (BPA gluc), and genistein 4′-β-D-glucuronide (genistein gluc) in biological matrices including urine, cultures and tissues using solid-phase microextraction (SPME), followed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). |
| Features and Benefits: | • Excellent reproducibility • Faster separation of strongly hydrophobic analytes than C18 columns • Stable, low-bleed LC-MS separations • Exceptional peak shapes for basic and acidic compounds • Compatible with low organic/highly aqueous mobile phases |
| Legal Information: | Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany |
| UNSPSC | 41115700 |