TSKgel® Phenyl-5PW HPLC Column
SUPELCO/807656 - phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size, stainless steel hardware
Synonym: TSKgel® Hydrophobic Interaction Chromatography (HIC) Column
Product Type: Equipment/Book
| L × I.D. | 15 cm × 21.5 mm |
| manufacturer/tradename | Tosoh Phenyl-5PW, 07656 |
| material | stainless steel hardware |
| matrix | polymer particle platform |
| matrix active group | phenyl phase |
| operating pH | 2-12 |
| parameter | ≤4 M salt concentration |
| ≤50% organic solvent | |
| 10-50 °C temperature | |
| 20 bar max. pressure | |
| 8 mL/min flow rate | |
| particle size | 13 μm |
| pore size | 1000 Å |
| product line | TSKgel® |
| separation technique | hydrophobic interaction (HIC) |
| technique(s) | HPLC: suitable |
| General description: | Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery. TSKgel BioAssist Phenyl columns contain the same particles as Phenyl-5PW columns, but are packed in PEEK column hardware rather than stainless steel. BioAsist Phenyl (Phenyl-5PW), TSKgel Ether-5PW and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing, such as BioAssist Phenyl. We recommend an Ether-5PW column for purifying very hydrophobic proteins. TSKgel BioAssist Phenyl and TSKgel Ether-5PW packings are based on TSKgel G5000PW resin - 10Â μm particles with 1000Â Ã… pores. TSKgel Butyl-NPR columns, which are less hydrophobic than Ether-5PW, is prepared from 2.5Â μm nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2Â M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins. |
| General description: | The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients. Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in water. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |