TSKgel® Phenyl-5PW RP HPLC Column
SUPELCO/814007 - phase phenyl, octadecyl, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
Product Type: Equipment/Book
extent of labeling | 1 meq/mL ligand density (estimate) |
L × I.D. | 7.5 cm × 8 mm |
manufacturer/tradename | Tosoh 14007 |
material | glass |
matrix | hydroxylated methacrylic polymer |
matrix active group | phenyl phase, octadecyl |
operating pH | 2-12 |
parameter | no limitation salt concentration |
0-100% organic solvent | |
2.5 mL/min flow rate | |
20 bar max. pressure | |
4-60 °C temperature | |
particle size | 10 μm |
product line | TSKgel® |
separation technique | reversed phase |
technique(s) | HPLC: suitable |
General description: | Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of strong basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel® columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. • Octadecyl-2PW particles have 125 A pores • Octadecyl-4PW particles have 500 Apores • Phenyl-5PW RP particles have 1000 A pores • Octadecyl-NPR particles are nonporous |
Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
Physical form: | Shipped in 30% methanol - 70% water. |
Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
UNSPSC | 41115711 |