TSKgel® Boronate-5PW HPLC Column
SUPELCO/814449 - phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
Synonym: TSKgel® Boronate-5PW HPLC Column
Product Type: Equipment/Book
| analyte chemical class(es) | proteins |
| capacity | 40 μmol/mL, resin adsorption capacity (sorbitol) |
| L × I.D. | 5 cm × 5 mm |
| manufacturer/tradename | Tosoh 14449 |
| material | glass |
| matrix | hydroxylated methacrylic polymer |
| matrix active group | m-aminophenyl boronate phase |
| operating pH | 2-9 |
| parameter | ≤20% organic solvent |
| ≤3 M salt concentration | |
| >0 °C temperature | |
| 1.2 mL/min flow rate | |
| 30 bar max. pressure | |
| particle size | 10 μm |
| product line | TSKgel® |
| separation technique | affinity |
| storage temp. | 2-8°C |
| technique(s) | HPLC: suitable |
| General description: | High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins. Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include: nucleic acids, catecholamines, nucleosides and nucleotides. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| Storage Temp. | 2-8°C |
| UNSPSC | 41115711 |