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TSKgel® Boronate-5PW HPLC Column

SUPELCO/814449 - phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass

Synonym: TSKgel® Boronate-5PW HPLC Column

Product Type: Equipment/Book
Catalog Number PKG Qty. Price Quantity
45-814449 1 ea
 $1830.00
1/EA		 Add To Favorites
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analyte chemical class(es) proteins
capacity 40 μmol/mL, resin adsorption capacity (sorbitol)
L × I.D. 5 cm × 5 mm
manufacturer/tradename Tosoh 14449
material glass
matrix hydroxylated methacrylic polymer
matrix active group m-aminophenyl boronate phase
operating pH 2-9
parameter ≤20% organic solvent
  ≤3 M salt concentration
  >0 °C temperature
  1.2 mL/min flow rate
  30 bar max. pressure
particle size 10 μm
product line TSKgel®
separation technique affinity
storage temp. 2-8°C
technique(s) HPLC: suitable
General description: High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Legal Information: TSKgel is a registered trademark of Tosoh Corporation
Other Notes: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
Storage Temp. 2-8°C
UNSPSC 41115711

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