TSKgel® Octadecyl-4PW HPLC Column
SUPELCO/816257 - phase C18 (octadecyl), L × I.D. 15 cm × 21.5 mm, 13 μm particle size
Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)
Product Type: Equipment/Book
| extent of labeling | 1 meq/mL ligand density (estimate) |
| L × I.D. | 15 cm × 21.5 mm |
| manufacturer/tradename | Tosoh 16257 |
| material | stainless steel hardware |
| matrix | polymer particle platform |
| matrix active group | C18 (octadecyl) phase |
| operating pH | 2-12 |
| parameter | no limitation salt concentration |
| 0-100% organic solvent | |
| 30 bar max. pressure | |
| 5-50 °C temperature | |
| 8 mL/min flow rate | |
| particle size | 13 μm |
| pore size | 500 Å |
| product line | TSKgel® |
| separation technique | reversed phase |
| technique(s) | HPLC: suitable |
| General description: | Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500Å pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations. |
| General description: | Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. • Octadecyl-2PW particles have 125Å pores • Octadecyl-4PW particles have 500Å pores • Phenyl-5PW RP particles have 1000Å pores • Octadecyl-NPR particles are nonporous |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in 60% methanol - 40% waters (v/v). |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |