TSKgel® Phenyl-5PW RP HPLC Column
SUPELCO/816260 - phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size
Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)
Product Type: Equipment/Book
| extent of labeling | 1 meq/mL ligand density (estimate) |
| L × I.D. | 15 cm × 21.5 mm |
| manufacturer/tradename | Tosoh Phenyl-5PW, 16260 |
| material | stainless steel hardware |
| matrix | polymer particle platform |
| matrix active group | phenyl phase |
| operating pH | 2-12 |
| parameter | no limitation salt concentration |
| 0-100% organic solvent | |
| 30 bar max. pressure | |
| 4-60 °C temperature | |
| 8 mL/min flow rate | |
| particle size | 13 μm |
| pore size | 1000 Å |
| product line | TSKgel® |
| separation technique | reversed phase |
| technique(s) | HPLC: suitable |
| General description: | Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. • Octadecyl-2PW particles have 125Å pores • Octadecyl-4PW particles have 500Å pores • Phenyl-5PW RP particles have 1000Å pores • Octadecyl-NPR particles are nonporous |
| General description: | TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in 30% methanol - 70% water. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |