TSKgel® Phenyl-5PW RP HPLC Column
SUPELCO/818756 - phase phenyl, L × I.D. 7.5 cm × 2 mm, 10 μm particle size
Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)
Product Type: Equipment/Book
| extent of labeling | 1 meq/mL ligand density (estimate) |
| L × I.D. | 7.5 cm × 2 mm |
| manufacturer/tradename | Tosoh Phenyl-5PW, 18756 |
| material | stainless steel hardware |
| matrix | polymer particle platform |
| matrix active group | phenyl phase |
| operating pH | 2-12 |
| parameter | no limitation salt concentration |
| 0-100% organic solvent | |
| 0.12 mL/min flow rate | |
| 10 bar max. pressure | |
| 4-60 °C temperature | |
| particle size | 10 μm |
| pore size | 1000 Å |
| product line | TSKgel® |
| separation technique | reversed phase |
| technique(s) | HPLC: suitable |
| General description: | Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. • Octadecyl-2PW particles have 125Å pores • Octadecyl-4PW particles have 500Å pores • Phenyl-5PW RP particles have 1000Å pores • Octadecyl-NPR particles are nonporous |
| General description: | TSKgel Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography. In contrast, TSKgel Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 A pore size accommodates globular protein samples up to 1,000,000 Da. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in 30% methanol - 70% water. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |