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TSKgel® BioAssist® Chelate HPLC Column

SUPELCO/820022 - phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardware

Synonym: TSKgel® Chelate-5PW HPLC Column and Guardgel Kit

Product Type: Equipment/Book
Catalog Number PKG Qty. Price Quantity
45-820022 1 ea
 $1960.00
1/EA		 Add To Favorites
extent of labeling 20 μmol/mL resin ligand density
L × I.D. 5 cm × 7.8 mm
manufacturer/tradename Tosoh 20022
material PEEK hardware
matrix polymer particle platform
matrix active group iminodiacetic acid phase
operating pH 2-12
parameter ≤20% organic solvent
  ≤3 M salt concentration
  ≤45 °C temperature
  1.2 mL/min flow rate
  10 bar max. pressure
particle size 10 μm
pore size 1000 Å
product line TSKgel®
separation technique affinity
technique(s) HPLC: suitable
General description: High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Legal Information: BioAssist is a registered trademark of Tosoh Corporation
Legal Information: TSKgel is a registered trademark of Tosoh Corporation
Other Notes: Discover LiChropur reagents ideal for HPLC  or LC-MS  analysis
UNSPSC 41115711

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