TSKgel® Amide-80 HPLC Column
SUPELCO/821487 - phase carbamoyl, L × I.D. 25 cm × 1 mm, 5 μm particle size
Synonym: TSKgel® Amide-80, 25 cm x 1 mm ID, 5 μm
Product Type: Equipment/Book
| agency | suitable for USP L68 |
| L × I.D. | 25 cm × 1 mm |
| manufacturer/tradename | Tosoh 21487 |
| material | stainless steel hardware |
| matrix | silica particle platform |
| matrix active group | carbamoyl phase |
| operating pH | 2.0-7.5 |
| parameter | 0-100% organic solvent |
| 10-80 °C temperature | |
| 120 bar max. pressure | |
| particle size | 5 μm |
| pore size | 100 Å |
| product line | TSKgel® |
| separation technique | hydrophilic interaction (HILIC) |
| surface area | 450 m2/g |
| technique(s) | HPLC: suitable |
| General description: | TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in 85% acetonitrile - 15% water. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |