TSKgel® SuperSW mAb HR Guard Column
SUPELCO/822857 - phase diol, L × I.D. 4 cm × 6 mm, 4 μm particle size
Synonym: TSKgel® Size Exclusion (SW-Type) HPLC Guard Column
Product Type: Equipment/Book
| agency | suitable for USP L20 |
| suitable for USP L59 | |
| L × I.D. | 4 cm × 6 mm |
| manufacturer/tradename | Tosoh SuperSW HR Guard, 22857 |
| material | stainless steel |
| matrix | silica particle platform |
| matrix active group | diol phase |
| operating pH | 2.5-7.5 |
| parameter | ≤0.5 M salt concentration |
| 0-100% aqueous soluble organic solvents | |
| 1 mL/min flow rate | |
| 10-30 °C temperature | |
| particle size | 4 μm |
| pore size | 250 Å pore size |
| product line | TSKgel® |
| separation technique | size exclusion (SEC) |
| technique(s) | HPLC: suitable |
| General description: | The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Physical form: | Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7. |
| Recommended products: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| UNSPSC | 41115711 |