TSKgel® Protein A-5PW HPLC Column
SUPELCO/823483 - 20 μm particle size, L × I.D. 3.5 cm × 4.6 mm
Product Type: Equipment/Book
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| L × I.D. | 3.5 cm × 4.6 mm |
| manufacturer/tradename | TSKgel® |
| material | PEEK |
| matrix | polymeric particle platform |
| matrix active group | Protein A phase |
| parameter | 20 bar pressure (290 psi) |
| 4-30 °C temperature | |
| particle size | 20 μm |
| product line | TSKgel® |
| separation technique | affinity |
| storage temp. | 2-8°C |
| technique(s) | HPLC: suitable |
| General description: | TSKgel Protein A-5PW is a 20 μm, 4.6 mm ID × 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain. An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH. |
| Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
| Other Notes: | Highlights • Wide dynamic range with high sample concentration load for mAb quantitation • Fast analysis: 1-2 min/analysis • High sensitivity for mAb determination • Long lifetime: .2,000 injections per column with no sign of deterioration |
| Other Notes: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| Storage Temp. | 2-8°C |
| UNSPSC | 41115709 |