TSKgel® Protein A-5PW HPLC Column
SUPELCO/823483 - 20 μm particle size, L × I.D. 3.5 cm × 4.6 mm
Product Type: Equipment/Book
L × I.D. | 3.5 cm × 4.6 mm |
manufacturer/tradename | TSKgel® |
material | PEEK |
matrix | polymeric particle platform |
matrix active group | Protein A phase |
parameter | 20 bar pressure (290 psi) |
4-30 °C temperature | |
particle size | 20 μm |
product line | TSKgel® |
separation technique | affinity |
storage temp. | 2-8°C |
technique(s) | HPLC: suitable |
Footnote: | Highlights • Wide dynamic range with high sample concentration load for mAb quantitation • Fast analysis: 1-2 min/analysis • High sensitivity for mAb determination • Long lifetime: .2,000 injections per column with no sign of deterioration |
General description: | TSKgel Protein A-5PW is a 20 μm, 4.6 mm ID × 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain. An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH. |
Legal Information: | TSKgel is a registered trademark of Tosoh Corporation |
Recommended products: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |